HiScript II U+ One Step qRT-PCR Probe Kit

January 12, 2022 Allan Craig 0 Comments

Properties

Quality level: 200

Use

  • enough for 100 reactions
  • enough for 20 reactions
  • enough for 500 reactions

Characteristic

  • dNTP included
  • hot start

Applications

  • DNA amplification: adequate
  • PCR: adequate
  • RT-PCR: adequate
  • RT-qPCR: adequate
  • cDNA synthesis: adequate
  • qPCR: adequate

Input: Purified RNA

Sent in: dry ice

Storage temperature: −20 ° C.

General description

The Hiscript II One-Step RT-qPCR Probe Kit is a convenient, ready-to-use kit with components optimized for reverse transcription and subsequent probe-based qPCR. The one-step format prevents contamination and allows for increased sensitivity. This kit is ideal for testing low-entry RNA samples.

Request

For use in real-time RT-PCR (or RT-qPCR) applications, including the detection of RNA in the samples being analyzed. The HiScript II One-Step RT-qPCR Probe Kit is a ready-to-use kit for reverse transcription and subsequent probe-based qPCR in a single tube. The kit’s HiScript II reverse transcriptase provides a reliable reverse transcription of RNA.

After reverse transcription, the hot-start version of Taq polymerase is activated at 95 ° C and HiScript II reverse transcriptase is simultaneously inactivated. In the sequential PCR reaction, the 5′-3 ′ exonuclease activity of the Taq polymerase cleaves the hybridized probe, separating the reporter from the quencher and releasing the fluorescent signal.

Features and Benefits

  • Ready-to-use, one-step RT-qPCR probe kit with all components for RT-qPCR (except primers, probes, and template RNA)
  • It uses the robust ABScript II reverse transcriptase (RT), which provides reliable and efficient reverse transcription on a wide range of RNA template amounts.
  • This one-step RT-qPCR kit improves sensitivity and effectively prevents contamination
  • Flexible format as the HiScript II One-Step RT-qPCR Probe Kit includes two separate 50X concentrations of ROX (Passive Reference) dye, which will be added as required by specific qPCR instruments.

Components

1. 2X One-Step RT-qPCR Probe Buffer *
2. HS Taq one-step probe
3. HiScript II-RT Enzyme Blend
4. 50X ROX Tint I *
5. 50X ROX II Tint **
6. RNase-free ddH2O